Intraspecific variability of leaf form and function across habitat types.

Trait-based ecology has already revealed main independent axes of trait variation defining trait spaces that summarize plant adaptive strategies, but often ignoring intraspecific trait variability (ITV). By using empirical ITV-level data for two independent dimensions of leaf form and function and 167 species across five habitat types (coastal dunes, forests, grasslands, heathlands, wetlands) in the Italian peninsula, we found that ITV: (i) rotated the axes of trait variation that define the trait space; (ii) increased the variance explained by these axes and (iii) affected the functional structure of the target trait space. However, the magnitude of these effects was rather small and depended on the trait and habitat type. Our results reinforce the idea that ITV is context-dependent, calling for careful extrapolations of ITV patterns across traits and spatial scales. Importantly, our study provides a framework that can be used to start integrating ITV into trait space analyses.

Trichoderma atroviride Enhances Impatiens walleriana Hook. f Growth and Flowering in Different Growing Media.

Trichoderma spp. are widely reported to regulate plant growth by improving nutrient uptake, photosynthesis, and abiotic stress tolerance. However, their possible application for bedding plants is little explored, especially when comparing different growing media. Considering that coconut coir dust is finding broader application in the ornamental plants sector as a peat substitute, this work was aimed to test the combination of Trichoderma atroviride AT10 and coconut coir dust on Impatiens walleriana plants. Four treatments were tested as a mix of: (i) two growing media (70:30), peat:perlite or coconut coir dust:perlite; and (ii) the absence or presence of a T. atroviride treatment. At the end of the production cycle, the biomass and ornamental parameters, leaf pigments, nutrient content of the plant tissues, and Trichoderma abundance were assessed. The results revealed that T. atroviride can readily colonize coir, and the same positive effects of inoculum were found in plants grown on both substrates. The biostimulant effect of T. atroviride was observed as an increase in the aboveground biomass, number and weight of flowers, pigments and nutrient concentration, thereby improving the commercial quality of I. walleriana. Thus, T. atroviride has shown its potential in making bedding plant cultivation more sustainable and improving the yield and aesthetic parameters of plants grown on peat and coconut coir dust substrates.

Study on the suitability of life cycle assessment for the estimation of donkey milk environmental impact.

In the last decades, western Countries increased their interest in innovative products like donkey milk and other activities carried out with donkeys (onotherapy, onotourism). Donkey milk is considered a high-added-value food and is very similar to human breast milk. It is also used as an ingredient in cosmetics. The growing public interest suggests the need for a pilot study on the sustainability of donkey milk production, according to Life Cycle Assessment (LCA) criteria. Milk was used as the Declared Functional Unit (DFU) and two different models were described, a Real Scenario Model (RSM, i.e. a farm with its declared milk yield), and an Increased Milk Production Model (IMPM, i.e., the same farm with theoretically increased milk yield). Allocation was applied both in RSM and IMPM; thus, different values of impact categories, i.e., Global Warming Potential (GWP, kg CO2 equivalents), Acidification Potential (ACP, g SO2 equivalents) and Eutrophication Potential (EUP, g PO43-) were observed. GWP improved after mass allocation and showed the lowest equivalents in IMPM, compared to economic and reference allocation criterion (P < 0.05). In RSM, allocations affected GWP in a different way: the smaller size of the DFU resulted in the largest estimation of CO2 equivalents (P < 0.05) for reference allocation, whereas the mass allocation estimates were lower than with economic allocation (P < 0.05). ACP and EUP followed the same trends. No differences were found in IMPM results across the three allocation methods used. Moreover, mass allocation values recorded in RSM did not significantly differ from IMPM. ACP and EUP of RSM improved after economic allocation, although they were less sustainable (P < 0.05) than all IMPM values and RSM equivalents after mass allocation (P < 0.05). As expected, the theoretical model with increased milk yield improved the sustainability of the system. Both scenarios were affected by allocation criteria. In RSM, the economic and mass allocations described a representative scenario where donkey meat contributed to subtracting equivalents from milk (the main product). The present paper is a pilot study estimating for the first time the environmental impact of donkey milk production, with the aim to stimulate further research.

Increased Cattle Feeding Precision from Automatic Feeding Systems: Considerations on Technology Spread and Farm Level Perceived Advantages in Italy.

Automation reduces the impact of farming on climate change and helps farmers adapt to its financial impact. Automatic feeding systems (AFSs) increase the ruminant's feeding precision and ease operators' workload. Such systems exist on a spectrum, requiring varied levels of operator support and installation complexity. A recent survey on farmers pointed out that those already running an AFS and those willing to buy one appreciate its ease of use, the resulting animal welfare, and the resulting overall benefits (increased production, farm profitability, and reduced feed waste). Whether technologically complex or not, studies have confirmed the benefits that farmers perceive to be underlying the remarkable reduction in the environmental impact of feeding operations (AFSs are electrically powered), the increase in animal welfare resulting from reduced conflicts around accessing the feed bunk, and the constant availability of fresh feed. However, their introduction should follow accurate and holistic structural and economic planning for existing and newly built facilities. The availability of public subsidies plays an essential role in pushing farmers to adopt the most modern digital technologies, whose benefits may even increase when farmers couple them with interconnected sensors to monitor animals' physiological states.

The Role of Inter- and Intraspecific Variations in Grassland Plant Functional Traits along an Elevational Gradient in a Mediterranean Mountain Area.

Elevational gradients offer special opportunities to investigate the relative role of intraspecific and interspecific trait variations in relation to stress gradients. We used an altitudinal gradient in the Mediterranean (Mt Velino, Central Italy) to study (1) how community-weighted means (CWM) and nonweighted means (CM) vary with elevation for plant height, specific leaf area, and seed mass; and (2) how variation patterns differ for inter- and intraspecific functional variability. We tested (1) if elevation influences community functional composition on the basis of the adaptive value of plant traits and (2) if the latter shows intraspecific variations according to the species' ability to cope with local conditions. We found that different traits showed different patterns, which can be linked to the function they express. Differences between communities were influenced more by differences between their traits (CM) than by the relative species coverage (CWM). Both highest and lowest elevations were the most selective due to their particularly severe climatic conditions. Intermediate elevations were the most favorable thanks to less constraining climatic conditions. Interspecific trait variability was the most relevant component, indicating a low plant ability to cope with environmental variations through phenotypic plasticity.

Functional composition and diversity of leaf traits in subalpine versus alpine vegetation in the Apennines.

Mediterranean high mountain grasslands are shaped by climatic stress and understanding their functional adaptations can contribute to better understanding ecosystems' response to global change. The present work analyses the plant functional traits of high-elevation grasslands growing in Mediterranean limestone mountains to explore, at the community level, the presence of different plant strategies for resource use (conservative vs. acquisitive) and functional diversity syndromes (convergent or divergent). Thus, we compared the functional composition and diversity of the above-ground traits related to resource acquisition strategies of subalpine and alpine calcareous grasslands in the central Apennines, a mountain region characterized by a dry-summer Mediterranean climate. We used georeferenced vegetation plots and field-measured plant functional traits (plant maximum height, specific leaf area and leaf dry matter content) for the dominant species of two characteristic vegetation types: the subalpine Sesleria juncifolia community and the alpine Silene acaulis community. Both communities are of particular conservation concern and are rich in endemic species for which plant functional traits are measured here for the first time. We analysed the functional composition and diversity using the community-weighted mean trait index and the functional diversity using Rao's function, and we assessed how much the observed pattern deviated from a random distribution by calculating the respective standardized effect sizes. The results highlighted that an acquisitive resource use strategy and relatively higher functional diversity of leaf traits prevail in the alpine S. acaulis community, optimizing a rapid carbon gain, which would help overcome the constraints exerted by the short growing season. The divergent functional strategy underlines the co-occurrence of different leaf traits in the alpine grasslands, which shows good adaptation to a microhabitat-rich environment. Conversely, in the subalpine S. juncifolia grassland, a conservative resource use strategy and relatively lower functional diversity of the leaf traits are likely related to a high level resistance to aridity over a longer growing season. Our outcomes indicate the preadaptation strategy of the subalpine S. juncifolia grassland to shift upwards to the alpine zone that will become warmer and drier as a result of anthropogenic climate change.

Activity budget, home range, and habitat use of moor macaques (Macaca maura) in the karst forest of South Sulawesi, Indonesia.

Primate behavior can be responsive to the different ecological pressures associated with different habitats, as well as to the effects of direct and indirect anthropogenic disturbance. The karst forest ecosystem of South Sulawesi (Indonesia) represents one of the few intact forests available for residual populations of the moor macaque, but our understanding of its habitat use is limited. In the present study, this gap in knowledge was addressed by observing the activity and habitat use of two groups of moor macaques and by assessing the suitability of different habitats in the karst forest. Through a fine-scale vegetation analysis of 1 ha of forest in Bantimurung Bulusaraung National Park, we identified the presence of two distinct habitats that differ in terms of forest structure and composition. The karst plain forest (KPF) provided a greater abundance and diversity of food resources than the karst tower forest (KTF). In addition, anthropogenic disturbance was high in the KPF but low in the KTF. Behavioral data collected via group scans indicate that the macaques devoted more time to feeding activities when in the KPF, suggesting an ability to adjust their feeding behavior to meet their nutritional needs. However, the larger of the two groups used the food-rich KPF more than expected, implying that the KTF may represent a valuable refuge for the smaller group, as it is a less risky portion of its home range. The results of this study therefore provide novel information on the ecology of moor macaques and their habitats that can inform conservation planning for remnant populations.

EVALUATION OF AGRICULTURAL TRACTOR SEAT COMFORT WITH A NEW PROTOCOL BASED ON PRESSURE DISTRIBUTION ASSESSMENT.

Professional drivers have been found to be at a high risk of developing low back pain due to prolonged sitting and vehicle vibration. In a previous survey carried out on 1,155 tractor drivers, tractor vibration and/or incorrect posture while driving were found to cause low back disorders in more than 80% of the interviewed drivers. In this context, the present research introduces a new evaluation protocol to assess the ergonomic characteristics of agricultural tractor seats through the use of pressure sensors, taking into account both static and dynamic conditions. The degree of comfort was defined by analyzing the pressure distribution exerted by a sample of 12 drivers sitting on two seats in five different operating conditions. The pressure distribution values thus obtained were compared with the corresponding pressures recorded with the 12 drivers sitting on a reference seat (rigid seat, backrest, and suspension) designed for the purpose. From the comparison, it was possible to define a comfort index (CI) that allowed an objective assessment of the two seats. Statistical analysis showed that the CIs of the two seats were significantly different in all five operating conditions. Moreover, the two seats showed different CIs as a function of the operating condition. Although less comfortable, one seat showed almost constant CI values in all five operating conditions. Conversely, the other seat showed a progressively decreasing CI from the static condition to the uneven ground condition.